Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Blood
Cell type
PBMC
Tissue
blood
Lineage
mesoderm
Description
peripheral blood mononuclear cells

Attributes by original data submitter

Sample

source_name
CLL,1228 Abexinostat 400mM 6h
tissue
Blood, PBMC
cell type
Human peripheral blood mononuclear cells
genotype
del 11q
disease state
CLL
chip antibody
BRD4 (Active Motif # 91301, 4ml)
treatment
Abexinostat

Sequenced DNA Library

library_name
GSM6666011
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 15 min and quenched with 0.125 M glycine. 15 ug of chromatin was immunoprecipitated per sample using antibodies against HDAC1 (Active Motif # 40967) and BRD4 (Active Motif # 91301, 4l), H3K9Ac (Active Motif # 39917), H3K27Ac (Active Motif # 39133), and RNA Pol II (Active Motif # 91151), Illumina sequencing libraries were prepared from the ChIP and input DNAs by the standard consecutive enzymatic steps of end-polishing, dA addition, and adaptor ligation. After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina's NextSeq 500.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
36058863
Reads aligned (%)
97.5
Duplicates removed (%)
24.3
Number of peaks
1334 (qval < 1E-05)

hg19

Number of total reads
36058863
Reads aligned (%)
96.9
Duplicates removed (%)
25.1
Number of peaks
1276 (qval < 1E-05)

Base call quality data from DBCLS SRA