Antigen

Antigen Class

TFs and others

Antigen

BRD4

Cell type

Cell type Class

Blood

Cell type

PBMC

Tissue

blood

Lineage

mesoderm

Description

peripheral blood mononuclear cells

Sample

source_name

CLL,1228 Abexinostat 400mM 6h

tissue

Blood, PBMC

cell type

Human peripheral blood mononuclear cells

genotype

del 11q

disease state

CLL

chip antibody

BRD4 (Active Motif # 91301, 4ml)

treatment

Abexinostat

Sequenced DNA Library

library_name

GSM6666011

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed with 1% formaldehyde for 15 min and quenched with 0.125 M glycine. 15 ug of chromatin was immunoprecipitated per sample using antibodies against HDAC1 (Active Motif # 40967) and BRD4 (Active Motif # 91301, 4l), H3K9Ac (Active Motif # 39917), H3K27Ac (Active Motif # 39133), and RNA Pol II (Active Motif # 91151), Illumina sequencing libraries were prepared from the ChIP and input DNAs by the standard consecutive enzymatic steps of end-polishing, dA addition, and adaptor ligation. After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina's NextSeq 500.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

36058863

Reads aligned (%)

97.5

Duplicates removed (%)

24.3

Number of peaks

1334 (qval < 1E-05)

hg19

Number of total reads

36058863

Reads aligned (%)

96.9

Duplicates removed (%)

25.1

Number of peaks

1276 (qval < 1E-05)